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Experience: None
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Part:BBa_K2150023:Design

Designed by: Jianyi Huang   Group: iGEM16_UCAS   (2016-10-12)


pCon+RBS+tetR+DT+pTet+RBS+T7RNP+Ter+RBS+pT7+GFP+DT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4379


Design Notes

This part is used for logic verification. Whether green fluorescence is observed serves as an indicator whether the system works. In real use, substitute GFP for target gene.


Source

pCon from BBa_J23119, RBS from BBa_B0034, tetR from BBa_C0040, DT from BBa_B0015,PTet from BBa_R0040, pT7 from BBa_K525998, GFP from BBa_E0040.

First terminator comes from BBa_B0013, while the second terminator from BBa_B0015.

T7RNAP is PCR amplified from genome of BL21.

References